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. 2013 Jan 8;14(1):1119–1131. doi: 10.3390/ijms14011119

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© 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Quantitative real-time PCR analysis of EsCRC transgenes in transgenic lines chosen for further phenotype analysis. (A) quantitative real-time PCR (qPCR) results showing EsCRC expression in the Arabidopsis crc-1 mutant transformed with 35S::EsCRC; (B) EsCRC expression in transgenic wild-type Arabidopsis; (C) qPCR based on relative expression analysis of EsCRC in transgenic tobacco. The expression of each gene was normalized against tubulin in Arabidopsis and actin in tobacco. Error bars represent SE for three replicate reactions.