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. 2013 Jan 14;14(1):1477–1501. doi: 10.3390/ijms14011477

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© 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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In vivo bioluminescence detection of luc expression 24 h after MM18/FA-PEG₅₇₀ diether (90:10; R = 1) lipoplexes nasal administration (A). Luminescence images were superimposed onto still images of each mouse using the associated software (WinLight 32, Berthold, Stuttgart, Germany, 2007). (B) In vivo transfection efficiency of MM18/FA-PEG₅₇₀ diether (90:10) based lipoplexes evaluated by luminescent measurements on trachea extracts Each mice received 50 μg of pDNA complexed or not. Results were expressed as RLU per mg of total proteins.