Table 1.
Flavin content analysis of Δ55AnCYPOR.
| Enzyme | FMN a | FAD a |
|---|---|---|
| Wild-type-Δ55AnCYPOR | 0.52 ± 0.01 | 0.64 ± 0.03 |
| L86F/L219F-Δ55AnCYPOR | 0.97 ± 0.02 | 0.56 ± 0.01 |
| L86F/L219F/C427R-Δ55AnCYPOR | 0.98 ± 0.01 | 0.82 ± 0.02 |
| L86F/L219F/W678A-Δ55AnCYPOR | 0.96 ± 0.04 | 0.55 ± 0.02 |
| L86F/L219F/W678H-Δ55AnCYPOR | 0.97 ± 0.02 | 0.57 ± 0.04 |
| Δ63AgCYPOR b | 0.72 ± 0.01 | 0.80 ± 0.01 |
| Human CYPOR b | 0.88 ± 0.01 | 0.92 ± 0.02 |
a
Flavin content is expressed as mean ± SD for mol of flavins per mol of protein in triplicate experiments. Standard flavin was measured and used for a plot of standard curve;
b
Lian et al., 2011 [18].