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. 2013 Feb 6;5(1):e00104. doi: 10.1042/AN20120048

Figure 5. TNFα increases ICAM-1 mRNA content in primary or immortalized PNMEC cultures.

Figure 5

(A) Primary PNMEC cultures were incubated in the absence (media) or presence of TNFα (10 ng/ml) for 3 h. (B, C) Immortalized PNMEC cultures were treated with (B) 0–100 ng/ml TNFα for 2 h or (C) 0–6 h with 10 ng/ml TNFα, as indicated. Relative changes in ICAM-1 mRNA content were quantified by qRT-PCR (quantitative RT-PCR). Data shown are the GAPDH-normalized fold changes from two separate experiments (n=4–9 cultures) and expressed as means ± S.E.M. #, P<0.05; unpaired Student's t-test. *, P<0.05; ** P<0.01; one-way ANOVA with Dunnett's post-hoc analysis.