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. 2013 Feb 6;5(1):e00104. doi: 10.1042/AN20120048

Figure 8. Secreted CCL2 promotes migration of THP-1 monocytes across PNMEC culture monolayers.

Figure 8

Immortalized PNMEC cultures were incubated in the absence (media) or presence of TNFα (10 ng/ml) for 4 h, and conditioned cell-culture medium was used to assay for THP-1 monocyte chemotaxis across PNMEC monolayers. (A) Total migrated cells in response to 2–6 h treatment with medium or TNFα-conditioned medium. (B) Total migrated cells in response to 4 h treatment with medium or TNFα-conditioned medium supplemented without (control) or with an CCL2 neutralizing antibody (α-CCL2) or with heat-denatured anti-CCL2 antibody. Data shown are the means±S.E.M. (n=3–7 cultures). (A) *, P<0.01, two-way ANOVA with Bonferroni's post-hoc analysis. (B) One-way ANOVA with Bonferroni's post-hoc analysis.