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. Author manuscript; available in PMC: 2013 Feb 6.
Published in final edited form as: Science. 2007 Jul 27;317(5837):513–516. doi: 10.1126/science.1144130

Fig. 1.

Fig. 1

NS3 unwinds DNA in three basepair steps. (A) PD1, a DNA with 18/20 double/single strand (ds/ss) was labeled with donor (Cy3), acceptor (Cy5) at the ds/ss junction and was tethered to a PEG surface by 3′ biotin. (B) Cy3 (green) and Cy5 (red) intensities monitored during unwinding of a single PD1 molecule. (raw time traces in light colors and 3 point averaged traces in dark colors) (C) Calculated FRET efficiency vs. time for the molecule shown in (B). (D,E) Two more example FRET traces of PD1. (F) PD2 is the same construct of 18/20 (ds/ss) prepared with dyes in the middle of duplex. (G–J) Equivalent plots to (B–D) for PD2.