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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Gastroenterology. 2012 Feb 28;142(7):1483–92.e6. doi: 10.1053/j.gastro.2012.02.040

Figure 1.

Figure 1

CAD is a NOD2-interacting protein. (A) HEK293T cells were transfected with Flag-CAD and HA-NOD2 constructs, MDP stimulated (100 ng/mL for 30 minutes) and lysates were immunoprecipitated with HA antibody, followed by immunoblot. (B) Same as in A except immunoprecipitation with Flag antibody. (C) 293:Flag-NOD2 cells were treated with MDP (100 ng/mL for 30 minutes) and lysates immunoprecipitated with Flag antibody or rabbit immunoglobulin G (IgG), followed by immunoblot. (D) HCT116 cells were MDP stimulated (10 µg/mL for 30 minutes) and lysates immunoprecipitated with CAD antibody or rabbit IgG, followed by immunoblot. (E) HEK293T cells were transfected with the indicated expression constructs and lysates immunoprecipitated with His antibody, followed by immunoblot.