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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Gastroenterology. 2012 Feb 28;142(7):1483–92.e6. doi: 10.1053/j.gastro.2012.02.040

Figure 5.

Figure 5

Specific regions of NOD2 and CAD mediate interaction and inhibition of NOD2. (A) Lysates from HEK293T cells transfected with Flag-CAD and HA-NOD2 deletion constructs were immunoprecipitated with Flag antibody followed by immunoblot. (B) Lysates from HEK293T cells transfected with HA-Nod2 and Flag-CAD constructs and MDP stimulated (100 ng/mL for 30 minutes) were immunoprecipitated with HA antibody followed by immunoblot. (C) NF-κB reporter assay in NOD2-expressing HEK293T cells cotransfected with indicated amounts of expression plasmids and MDP stimulated (10 ng/mL for 18 hours). Luciferase values were normalized to β-galactosidase transfection control values (nLuc), and averages±SD are shown. (D) MAPK p38 reporter assay in NOD2-expressing HEK293T cells cotransfected with indicated expression plasmids (100 ng) and MDP stimulated (100 ng/mL for 18 hours). Reporter activity assessed as in C. (E) Gentamicin protection assay in HCT116 cells transfected with indicated expression plasmids and MDP stimulated (10 µg/mL) during infection. Averages ±SD are shown. **P < .01, ***P < .001.