FIG. 5.

Quantitative reverse transcription (RT)-polymerase chain reaction analysis showing the expression levels of transgenes and endogenous genes IMR90 hiPSC lines. (i) Oct4; (ii) Sox2; (iii) Nanog; and (iv) Rex1 in single-allele biallele CCR5-modified hiPSCs, respectively, before and after the removal of reprogramming transcription factor genes cassette using Cre-recombinase. Expression levels of individual transcripts (Oct4, Sox2, Nanog and Rex1) were normalized to glyceraldehyde 3-phosphate dehydrogenase expression. IMR90 cells, which were used as a control, did not reveal expression of any pluripotency genes from their genomic loci. Fib denotes IMR90 cells; S-hiPSCs denotes single-allele CCR5-modified hiPSC lines; and B-hiPSCs denotes biallele CCR5-modified hiPSC lines.