Fig. 2.

Stacked confocal microscopy images illustrating labeled cells in the granular cell layer of the hippocampus (A–E) and amygdala (AMY) (F–I). Cells were labeled for BrdU (red), the mature neuronal marker NeuN (green), or both (yellow). The white box in Panel A indicates the area of the DG with high magnification (B–D; stack thickness=10 μm). Arrows indicate cells double-labeled for BrdU/NeuN. Arrowheads indicate the double-labeled cell shown in the magnified image in the DG (E) and AMY (I; stack thickness =8 μm). 3D co-localization of BrdU and NeuN is demonstrated using views along the y–z axis (right) and x–z axis (below). Scale bar=20 μm (A–D and F–H) or 5 μm (E and I). (J) Isolated females (n=8) had significantly lower percentages of BrdU-ir cells that co-labeled with NeuN in the hippocampal granular cell layer (GCL) and AMY, compared to control females (n=10). *p<0.05. Error bars represent SEM.