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. Author manuscript; available in PMC: 2013 Feb 6.
Published in final edited form as: Chromosome Res. 2011 Apr 12;19(4):457–470. doi: 10.1007/s10577-011-9208-5

Figure 3. Human chromosome X (HSAX) alpha satellite (DXZ1) array size compared to CENP-A chromatin domain size.

Figure 3

(a) DXZ1 arrays (light gray bars) were measured on different HSAXs present in human diploid (DIP) male lines or rodent-human somatic cell hybrids (SCX) in which HSAX was the only human chromosome. CENP-A chromatin domain size in megabases (dark gray bars) was determined using CENP-A immunostaining and FISH with a DXZ1 probe. DXZ1 arrays and CENP-A domain sizes varied up to three-fold among individual HSAXs. Error bars represent standard deviations in the genomic length (in megabases). (b) Calculation of the percentage of DXZ1 occupied by the CENP-A domain (dark gray bars) showed that the CENP-A domain was proportionally assembled on DXZ1 arrays (light gray bars) that varied in size among different HSAXs. Thirty-five to forty-five percent of DXZ1 was occupied by CENP-A chromatin. Error bars represent standard deviations in the percentage of DXZ1 occupied by the CENP-A domain.