Figure 1.

(a) Phylogenetic reconstruction of cry2 in holometabolic insects. The ML tree is constructed from full-length coding sequences of cry2. Bootstrap values are shown at the nodes. The branches tested for positive selection are labelled as a–g. Asterisk (*) indicates that positively selected sites are detected. The species that lack cry1 are highlighted in blue. (b) Phylogeny of the holometabolic insects, character mapping of possession of cryptochrome genes and proposed ancestral state. Triangles indicate cry1 (red) and cry2 (blue).