Fig. 3. Selective Tachykinin NK1 receptor antagonists attenuate repeated opioid-mediated augmentation of spinal TNFα immunoreactivity in rats.
After drug treatment and behavioral test, the animals were euthanized and their spinal cords were isolated. Lumbar spinal cord segments were homogenized and TNFa immunoreactivty was measured using a Rat TNFα ELISA kit.