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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Circ Res. 2012 Dec 18;112(3):432–440. doi: 10.1161/CIRCRESAHA.112.300755

Figure 4. FLAP deficiency attenuates VSMC migration by disruption of LTB4 and proinflammatory cytokine synthesis.

Figure 4

Figure 4

A, Peritoneal macrophages from WT or FLAP KO mice were cultured and stimulated with A23187 (10μM) for 20 minutes or LPS (5μg/ml) overnight. 5HETE, LTB4, LTC4, LTD4 and LTE4were measured using cell culture supernatant. **P<0.01, n=5. B, Macrophage cell culture supernatants were collected at 0 hour and 12 hours after LPS stimulation. TNF-α and IL-6 levels were measured by ELISA. * p<0.05, n=5–10. C, Vascular SMCs isolated from the aortas of WT were placed in modified Boyden chambers and chemotaxis assessed to stimulated peritoneal macrophage medium harvested from either WT or FLAP KO mice as indicated. As noted, LTB4 or LTD4 are added into the lower wells. * p<0.05, **P<0.01, n=3.