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. Author manuscript; available in PMC: 2013 Feb 6.
Published in final edited form as: Infect Genet Evol. 2011 Sep 23;11(8):2011–2019. doi: 10.1016/j.meegid.2011.09.010

Figure 3. Histogram of the Shannon Entropy in the DENV-3 NS5 Protein by Time of Isolation.

Figure 3

A stacked column graph showing the Shannon entropy scores calculated for each amino acid position in the DENV-3 NS5 protein. Subsets of sequences were divided based on time and/or place of isolation and include: the Asian sequences isolated from Thailand in 2001 (Asia), and various sequences isolated from Venezuela during the first DENV-3 outbreak in 2001 (Ven 2001), 2002-2006 (Ven 2002-2006), the second DENV-3 outbreak in 2007-2008 (Ven 2007-2008), and all Venezuelan DENV-3 sequences combined (Venezuela All). The NS5 G374E mutation occurred between the 2001 and 2007-2008 outbreaks and has been associated with viral resistance to the host interferon response. The height of each color within each peak represents the Shannon entropy for that sequence set alone at the specified position in the NS5 protein. The height of each peak represents the amount of amino acid variability for each individual position in all sequence sets for the NS5 mature protein. Any column that is conserved in the multiple sequence alignment will not have an entropy score.