Table 2.
Primers used for amplification and sequencing of the GBA gene
| Primer | Sequence | Amplicon size | Template |
|---|---|---|---|
| GBA-XL1F | CTGGGATTTAGGAGCCTCTACC | 3493 | Genomic DNA |
| GBA-XL1R | AAGATTGACAGGCCCAAGGC | ||
| GBA-XL2F | TCAAATGATATACCTGCCTTGG | 2045 | Genomic DNA |
| GBA-XL2R | AGTTTGGGAGCCAGTCATTT | ||
| GBA-XL3F | AAGTTCCAGAAGCCTGTGTGC | 3992 | Genomic DNA |
| GBA-XL3R | CAGGACCTTGACGGCCAGCG | ||
| GBA-E01F | TCACCCATACATGCCCTCCA | 378 | Amplicon XL1 |
| GBA-E01R | TCCAGTGCCAGGATTCCAGA | ||
| GBA-E02F | CCAGGTTTCTGTGGGCCTTG | 291 | Amplicon XL1 |
| GBA-E02R | ACCAAAGGACTATGAGGCAGAAGG | ||
| GBA-E03-04F | GGCCAAGGGGTGAGGAATTT | 649 | Amplicon XL1 |
| GBA-E03-04R | GCCGACAGAATGGGCAGAGT | ||
| GBA-E05F | GACTGGCAAGTGATAAGCAGAGTCC | 356 | Amplicon XL2 |
| GBA-E05R | ATCCGGTTCAGCCATTAGCC | ||
| GBA-E06F | GCTAATGGCTGAACCGGATGC | 413 | Amplicon XL2 |
| GBA-E06R | AAGATTGACAGGCCCAAGGCTG | ||
| GBA-E07F | TCCTGAACTCAAGTGATCCACC | 432 | Amplicon XL2 |
| GBA-E07R | TTTGGGAGCCAGTCATTTGG | ||
| GBA-E08F_PCR | CAAAATCTCCCCAAACCTCTCTAG | 1358 | Genomic DNA |
| GBA-E08R_PCR | GTGCCACTGCGTCCAGGT | ||
| GBA-E08F_CYC | ACTAGAAGTTCCAGAAGCCTG | ||
| GBA-E08R_CYC | CTTGGTGATGTCTACAATG | ||
| GBA-E09F | TGCCTCTCCCACATGTGACC | 374 | Amplicon XL3 |
| GBA-E09R | TGCCTCTTCCGAGGTTCCAC | ||
| GBA-E10F | GTCCGTGGGTGGGTGACTTC | 322 | Amplicon XL3 |
| GBA-E10R | TTGATGGTAAGAGGCACATCCTTAG | ||
| GBA-E11F | ACTTCTTAGATGAGGGTTTCATG | 465 | Amplicon XL3 |
| GBA-E11R | TTTAGTCACAGACAGCGTGTG |