Table 1.
Mutation | Primer sequence (5´ > 3´) | Probe sequence (5´ > 3´) |
---|---|---|
N370S | Forward | Normal |
GCCTTTGTCTCTTTGCCTTTGTC | TTACCCTAGAACCTCCTG – VIC | |
Reverse | Mutant | |
CCAGCCGACCACATGGTA | ACCCTAGAGCCTCCTG – FAM | |
L444P | Forward | Normal |
CTGAGGGCTCCCAGAGAGT | CTGCGTCCAGGTCGT – VIC | |
Reverse | Mutant | |
GCCATCGGGATGCATCAGT | TGCGTCCGGGTCGT – FAM |