Table 2.
Primers used to amplify and sequence the GBA gene
| Exons and flanking regions | Primer sequence (5´ > 3´) | Tannealing
(0C) |
Length (bp) |
|
|---|---|---|---|---|
| Forward | Reverse | |||
| 1 | CCTAGTGCCTATAGCTAAGG | CTGGATTCAAAGAGAGTCTG | 57 | 236 |
| 2 | GTCCTAATGAATGTGGGAGACC | CTTACTGGAAGGCTACCAAAGG | 61 | 286 |
| 3 | GTTCAGTCTCTCCTAGCAGATG | GGAAACTCCATGGTGATCAC | 61 | 353 |
| 4 | GTCCTCCTAGAGGTAAATGGTG | GCAGAGTGAGATTCTGCCTC | 61 | 316 |
| 5 | GATAAGCAGAGTCCCATACTCTC | CTGTACAAGCAGACCTACCCTAC | 62 | 281 |
| 6 | CTAATGGCTGAACCGGATG | GGAAGTGGAACTAGGTTGAGG | 62 | 346 |
| 7 | CAAAGTGCTGGGATTACAGG | CTCTAAGTTTGGGAGCCAGTC | 62 | 404 |
| 8 | CTAGTTGCATTCTTCCCGTC | GCTTCTGTCAGTCTTTGGTG | 63 | 407 |
| 9 | CTCCCACATGTGACCCTTAC | CTCGTGGTGTAGAGTGATGTAAG | 61 | 329 |
| 10 and 11 | GTGGGTGACTTCTTAGATGAGG | CTTTAGTCACAGACAGCGTGTG | 62 | 473 |