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SDS–PAGE analysis of chimMotB purification. L1: total cell lysate, soluble fraction; L2: flow-through, Ni–NTA; L3: eluate during 80 mM imidazole wash, Ni–NTA; L4 eluate during 2 mM MgATP wash, Ni–NTA; L5 Ni–NTA eluate; L6: pooled peak fractions from gel-filtration step; L7: omission of the 2 mM MgATP washing step resulted in a higher level of DnaK contamination.
