Figure 4.

Tests examining the necessity of PCR-amplification of DNA samples, using the LTS platform. PCR and genomic DNA screenings without the use of tagged beads, i.e. only oligonucleotide primers. Both purified samples and sample + background (non-target) DNA are evaluated. (a) Comparison of background PCR product (black) and background PCR product plus quagga-specific tags (red). (b) PCR product screening: black line represents quagga PCR product with no tags; red line represents quagga PCR product only plus species-specific tags; yellow line represents quagga PCR product + background PCR product with tags. (c) Background DNA (black) and background DNA plus quagga-specific tags (red). (d) Genomic DNA screening: black line represents quagga DNA with no tags; red line represents quagga DNA only plus species-specific tags; yellow line represents quagga DNA + background DNA with quagga-specific tags.