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NetB in planar lipid bilayers. (A) Representative single-channel events recorded after addition of NetB (2 µg) to a PE-PS bilayer bathed in 0.1 M KCl and held at +40 mV. (B and C) Expanded time scales of two sections of panel A. Note the variation in closed levels in panel B and the small “flickery” events of 50 to 90 pS in panel C. (D) Distribution of mean conductance values obtained for NetB. (E) Effect of ion replacement on mean conductance values.
