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. 2013 Feb 5;4(1):e00033-13. doi: 10.1128/mBio.00033-13

TABLE 1.

IlvE activity is decreased in a ridA strain by serine/threonine dehydratasesa

Relevant genotype IlvE sp act (nmol 2KMV ⋅ min−1
 mg−1 protein) in indicated medium
Minimal Minimal + isoleucine
ridA+ pSU18 (empty) 73 ± 9 52 ± 2
ridA pSU18 (empty) 18 ± 5 51 ± 11
ridA+ pSU18-tdcB+ 72 ± 8 77 ± 6
ridA pSU18-tdcB+ 2 ± 4 6 ± 3
a

Cells were grown in the indicated media, and IlvE activity was measured in permeabilized cells as described in Materials and Methods. IlvE specific activity is expressed in nmol 2KMV ⋅ min−1 mg−1 protein; shown are averages and standard deviations of results from three biological replicates.