TABLE 1.
Relevant genotype | IlvE sp act (nmol 2KMV ⋅ min−1 mg−1 protein) in indicated medium |
|
---|---|---|
Minimal | Minimal + isoleucine | |
ridA+ pSU18 (empty) | 73 ± 9 | 52 ± 2 |
ridA pSU18 (empty) | 18 ± 5 | 51 ± 11 |
ridA+ pSU18-tdcB+ | 72 ± 8 | 77 ± 6 |
ridA pSU18-tdcB+ | 2 ± 4 | 6 ± 3 |
Cells were grown in the indicated media, and IlvE activity was measured in permeabilized cells as described in Materials and Methods. IlvE specific activity is expressed in nmol 2KMV ⋅ min−1 mg−1 protein; shown are averages and standard deviations of results from three biological replicates.