FIG 4 .

Specific inhibition of LSD1 by OG-L002 but not treatment with an MAO inhibitor results in accumulation of repressive chromatin on viral IE gene promoters. HeLa cells were pretreated with DMSO, OG-L002 (50 µM), clorgyline (50 µM), or TCP (2 mM) for 4 h, followed by infection with HSV-1 (0.1 PFU/cell) for 3 h. ChIP assays were performed using control IgG, H3K9-me2, or histone H3 antibodies. The levels of H3K9-me2 and histone H3 associated with viral IE promoters (ICP0 and ICP27) and cellular controls (RPL and SATa) are expressed as the percentage of input. RPL is a transcriptionally active cellular gene, and SATa is a transcriptionally repressed cellular region. The data shown are representative of two independent experiments.