FIG 7 .

Inhibition of LSD1 by novel inhibitor OG-L002 results in reduced expression of hCMV IE genes and adenovirus E1A gene. (A) MRC-5 cells were treated with control DMSO or the indicated concentrations (micromolar) of OG-L002 for 3 h, followed by infection with hCMV (0.1 PFU/cell) for 5 h. The mRNA levels of the viral IE genes (IE72, UL37, and US3) and control genes (Sp1, GAPDH, and TBP) were determined by qRT-PCR and expressed as ratios to the levels in DMSO-treated cells. (B) HeLa cells were pretreated with control DMSO, OG-L002 (50 µM), or clorgyline (50 µM) for 4 h, followed by infection with adenovirus 5 (5.6 × 10³ normalized infectious units [NIU]/cell) for 2 h. The mRNA levels of the viral E1A gene and cellular control genes (S15 and TBP) were determined by qRT-PCR and expressed as ratios to the levels of the DMSO control.