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. Author manuscript; available in PMC: 2013 Feb 6.
Published in final edited form as: Sci Signal. 2012 Jul 3;5(231):ra47. doi: 10.1126/scisignal.2002712

Fig. 2.

Fig. 2

Impact of perinuclear mitochondrial clustering on hypoxia-induced pan-cellular ROS generation. (A) Pseudo-colored intensity plots of roGFP signal in normoxic PAECs and PAECs cultured in hypoxia (H) or cultured in hypoxia in the presence of nocodazole or after siRNA-mediated dynein knockdown (siDYN). roGFP signal intensity (indicated by the color bar) correlates with ROS concentrations. Scale bar, 15 μm. (B) Quantitative assessment of nuclear and cytoplasmic roGFP signals in the absence and presence of nocodazole (Noc) in normoxic (N) and hypoxic (H) PAECs. (C) Effect of dynein heavy chain–specific siRNA (siDYN) or scrambled siRNA (Scram) on hypoxia-induced changes in nuclear and cytoplasmic roGFP signals. n = 3 to 5 different culture dishes with three to six cells analyzed per dish for all panels. *P < 0.05, increased from normoxia.