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Indirect immunofluorescence assay of replicon particle (RP) infected Vero cells. Vero cell monolayers were infected with RP expressing either (A) the E2 glycoprotein gene of BVDV subgenotype 1b strain NY1 or (B) a non-BVDV-derived gene as a control. Cells were fixed and subsequently stained with a monoclonal antibody specific to BVDV 1b E2 and a fluorescent-conjugated anti-mouse secondary antibody.
