Table 1.
Origin of oxygen in the hydroxylation and oxidation products of benzene and phenol formed by AaeAPO in the presence of H218O2
| Substrate | Oxygen atoms in hydroquinone [rel.%] | Oxygen atoms in p-benzoquinone [rel.%] | Oxygen atoms in catechol [rel.%] | ||||||
|---|---|---|---|---|---|---|---|---|---|
| |
16O/16O |
16O/18O |
18O/18O |
16O/16O |
16O/18O |
18O/18O |
16O/16O |
16O/18O |
18O/18O |
| Benzene |
n.d.§ |
n.d.§ |
n.d.§ |
20 |
55 |
25 |
100 |
0 |
0 |
| Benzene# |
9 |
25 |
66 |
n.d.§ § |
n.d.§ § |
n.d.§ § |
18 |
31 |
51 |
| Phenol |
42 |
58 |
0 |
42 |
58 |
0 |
100 |
0 |
0 |
| Phenol# | 3 | 97 | 0 | n.d.§ § | n.d.§ § | n.d§§ | 6 | 94 | 0 |
# in presence of ascorbic acid (4 mM).
§ n.d. – not detectable, the concentration was too low to give a viable LC-MS signal.
§§ n.d. – not detected in the presence of ascorbic acid.