. 2013 Jan 28;3:7. doi: 10.1186/2191-0855-3-7

Table 1.

Oligonucleotide primers used for PCR amplifications in this study

Primer name	Restriction enzyme^c	Sequence (5′-3′)	Target gene
V1^a	-	ATGGATCAGAAGAATAAGCTTTC	dkr
V2^b	SpeI	TCTACGACTAGTTCAGTTCAGATCATTCGG	dkr
V3^b	XhoI	CTATCGCTCGAGTCAGTTCAGATCATTCGGG	dkr
P1^a	SpeI	CGGAAATCACGGGAACTAGTCGCCAAA	P_sppA, P_sppQ
P2^b	-	CGGTACCTACAACAGACATGGGAATCATACTCCTATATATTATT	P_sppA
P3^b	-	CGGTACCCACAACAGACATATATGCTGGCCAGCTAAGTA	P_sppQ

^a forward primer.

^b reverse primer.

^c restriction sites are underlined.