Table 3.
Not pH regulated cultivations of L. lactis and Lb. plantaruma | |||||
---|---|---|---|---|---|
Strain /plasmid |
Volumetric activity (U L-1fermentation broth) |
Specific activity (U mg-1protein) |
Induction factorb |
||
Induced | Noninduced | Induced | Noninduced | ||
L. lactis NZ3900/pVK51dkr |
81.6 ± 9.3 |
16.0 ± 0.24 |
0.191 ± 0.022 |
0.038 ± 0.001 |
5.0 |
Lb. plantarum/pSIP603dkr |
102 ± 5.8 |
21.7 ± 2.0 |
0.232 ± 0.022 |
0.054 ± 0.002 |
4.3 |
Lb. plantarum/pSIP609dkr |
104 ± 2.75 |
23.8 ± 2.2 |
0.243 ± 0.017 |
0.055 ±0.001 |
4.4 |
pH regulated cultivations (pH 6.5) of L. lactis and Lb. plantaruma | |||||
Strain /plasmid |
Volumetric activity (U L-1 fermentation broth) |
Specific activity (U mg-1protein) |
Induction factor b |
||
|
Induced |
Noninduced |
Induced |
Noninduced |
|
L. lactis NZ3900/pVK51dkr |
114 ± 1.9 |
14.6 ± 2.0 |
0.188 ± 0.001 |
0.022 ± 0.003 |
8.5 |
Lb. plantarum/pSIP603dkr |
226 ± 5.9 |
27.2 ± 0.56 |
0.264 ± 0.026 |
0.032 ± 0.001 |
8.3 |
Lb. plantarum/pSIP609dkr | 262 ± 1.7 | 30.2 ± 1.2 | 0.308 ± 0.016 | 0.033 ± 0.004 | 9.3 |
a All data are mean values of three independent experiments. The maximum volumetric and specific activities measured in the culture are presented.
b The induction factor was defined as the ratio between the specific activity obtained under induced conditions and the activity obtained under noninduced conditions.