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. 2013 Jan 28;3:7. doi: 10.1186/2191-0855-3-7

Table 3.

Maximum 2,5-DKG reductase activities in cell free extracts of induced and noninduced L. lactis NZ3900 and Lb. plantarum TLG02 cultures

Not pH regulated cultivations of L. lactis and Lb. plantaruma
Strain /plasmid
Volumetric activity (U L-1fermentation broth)
Specific activity (U mg-1protein)
Induction factorb
  Induced Noninduced Induced Noninduced  
L. lactis NZ3900/pVK51dkr
81.6 ± 9.3
16.0 ± 0.24
0.191 ± 0.022
0.038 ± 0.001
5.0
Lb. plantarum/pSIP603dkr
102 ± 5.8
21.7 ± 2.0
0.232 ± 0.022
0.054 ± 0.002
4.3
Lb. plantarum/pSIP609dkr
104 ± 2.75
23.8 ± 2.2
0.243 ± 0.017
0.055 ±0.001
4.4
pH regulated cultivations (pH 6.5) of L. lactis and Lb. plantaruma
Strain /plasmid
Volumetric activity (U L-1 fermentation broth)
Specific activity (U mg-1protein)
Induction factor b
 
Induced
Noninduced
Induced
Noninduced
 
L. lactis NZ3900/pVK51dkr
114 ± 1.9
14.6 ± 2.0
0.188 ± 0.001
0.022 ± 0.003
8.5
Lb. plantarum/pSIP603dkr
226 ± 5.9
27.2 ± 0.56
0.264 ± 0.026
0.032 ± 0.001
8.3
Lb. plantarum/pSIP609dkr 262 ± 1.7 30.2 ± 1.2 0.308 ± 0.016 0.033 ± 0.004 9.3

a All data are mean values of three independent experiments. The maximum volumetric and specific activities measured in the culture are presented.

b The induction factor was defined as the ratio between the specific activity obtained under induced conditions and the activity obtained under noninduced conditions.