Table 1. Kinetic parameters of fumarase and tartrate dehydratase activity of FumA and FumB^a.

	L-malate			fumarate				D-tartrate
	b Km	c Vmax	d kcat/Km	Km	Vmax	kcat/Km	e Keq	Km	Vmax	kcat/Km
FumA	0.7±0.1	(7.2±0.4)·102	(1.0±0.2)⋅106	0.46±0.08	(19±1)·102	(4.2±0.8)⋅106	4±1	0.8±0.3	2.3±0.3	(3±1)⋅103
FumB	0.30±0.07	(4.9±0.3)·102	(1.6±0.4)⋅106	0.32±0.04	(14.3±0.6)·102	(4.5±0.6)⋅106	2.8±0.8	0.8±0.1	9.2±0.4	(12±2)⋅103

^aAll values are corrected for Fe-S cluster content.

^b K_m in mM.

^c V_max in µmol product/minute/mg enzyme.

^d k_cat/K_m in s⁻¹ M⁻¹.

^e K_eq is the equilibrium constant for the hydration of fumarate as calculated using the Haldane relationship: K_eq = (k_cat/K_M)_fumarate/(k_cat/K_M)_L-malate.