Figure 5. Survivin expression and anoikis susceptibility in colorectal cancer cells.

A. Subcellular fractionation and immunoblot analysis of Survivin. The cell lysate from exponentially growing cells was fractionated into the detergent-soluble cytoplasmic (C), and nuclear (N) fractions and the detergent-insoluble pellet (P) fraction for immunoblot analysis with anti-Survivin, anti-α-tubulin, anti-H3-histone, and anti-β-actin. The detergent-soluble cytoplasmic Survivin level was estimated by immunoblot analysis, and expressed as a percentage of the level of 8505C, which is undifferentiated thyroid carcinoma showing anoikis resistance (+++, >80%; ++, 40–80%; +, 10–40%; −, <10%). B. Anoikis susceptibility in in colorectal cancer cells. Cells were suspended in serum-free medium for 72 h. Cell viability was determined by WST-1 assay.