Figure 3. Effect of 2MeSAMP on PAR4 enhancing intracellular Ca²⁺ mobilization in mouse platelets.

Fura 2-loaded wild type (black) and PAR3^−/− (gray) platelets were incubated at 37°C for 5 min in the absence or the presence of 100 µM 2MeSAMP. After treatment, platelets were activated 100 nM thrombin (A,) or 2 mM AYPGKF (B) for 10 min at 37°C in the presence of 2 mM of CaCl₂. The difference between the maximum increase and the basal intracellular Ca²⁺ mobilization was measured. The results are the mean (± SD) of three independent experiments (* p<0.05).