Figure 3. cPLA2 activation under PA supplementation.

(A) Western blot analyses of the effects of PA treatment on cPLA₂ protein activation in its phosphorylated form in NB100 cells. Cells were examined after treatment for 15–180 min with 150 µM PA. Cell lysates were resolved by SDS/PAGE. Proteins were blotted and detected with monoclonal antibodies against cPLA₂ or phospho-cPLA₂. Representative gels are shown. (B) The bar graph represents the band intensity values obtained by the Image J analysis, expressed as percentage of the corresponding control (Time 0) (white bars: total cPLA₂; black bars: phospho-cPLA₂). AA quantity in membranes as % of control (grey bars) is obtained from Table 1. Results are mean ± SD of at least three independent experiments. Phospho-cPLA₂ is significantly higher than controls at 15, 30 and 60 min (p<0.0001). AA is significantly higher at 30 min and significantly lower at 180 min (p = 0.001).