Table 3. Membrane phospholipid fatty acids of NB100 cells treated for the indicated times with 150 µM PA, 50 µM OA, 50 µM AA and compared to controls grown in the same conditions without fatty acid supplementation.
| FAMEa | Controlb | 30′b | 1 hb | 1 h30′b | 2 hb | 2 h30′b | 3 hb |
| (n = 6) | (n = 4) | (n = 4) | (n = 4) | (n = 4) | (n = 4) | (n = 6) | |
| 16∶0 | 25.5±1.3 | 29.0±3.3 | 32.1±0.3 c | 33.3±0.9 c | 33.1±3.0c | 29.8±3.1 | 37.1±2.6c |
| 9c-16∶1 | 5.6±0.1 | 3.9±0.9d | 4.4±0.2 d | 4.2±0.3 d | 3.9±0.1d | 4.0±0.0d | 3.9±0.2d |
| 18∶0 | 14.4±0.8 | 13.3±0.6 | 12.1±0.6d | 10.9±0.2d | 9.3±0.6d | 8.7±0.6d | 8.6±0.1d |
| 9t-18∶1 | 0.2±0.0 | 0.2±0.0 | 0.2±0.0 | 0.2±0.0 | 0.2±0.0 | 0.2±0.0 | 0.2±0.0 |
| 9c-18∶1 | 32.5±0.1 | 29.6±1.5d | 28.1±0.4d | 27.3±0.8d | 28.0±1.4d | 29.1±1.4d | 25.8±1.0d |
| 11c-18∶1 | 6.5±0.2 | 5.5±0.2d | 4.9±0.1d | 4.6±0.4d | 4.3±0.1d | 4.3±0.1d | 3.8±0.2d |
| 9c,12c-18∶2 | 3.9±0.5 | 3.2±0.2f | 2.8±0.2d | 3.7±1.3 | 3.4±0.2 | 3.2±0.0d | 2.7±0.1d |
| 8c,11c,14c-20∶3 | 1.0±0.1 | 0.7±0.1f | 0.8±0.1f | 0.7±0.1f | 0.7±0.1f | 0.8±0.1 | 0.7±0.1f |
| 5c, 8c,11c,14c-20∶4 | 5.9±0.2 | 10.8±1.4c | 11.6±0.5c | 12.5±0.3c | 14.1±1.4c | 16.5±1.9c | 14.8±0.8c |
| trans-20∶4* | 0.1±0.0 | 0.1±0.0 | 0.1±0.0 | 0.1±0.0 | 0.1±0.0 | 0.1±0.0 | 0.1±0.0 |
| SFA | 39.9±0.4 | 42.2±2.7 | 44.2±0.3c | 44.1±0.7c | 42.4±3.6 | 38.5±3.7 | 45.7±2.5 |
| MUFA | 44.6±0.4 | 39.0±0.9d | 37.3±0.7d | 36.1±1.6d | 36.2±1.6d | 37.4±1.4d | 33.5±1.4d |
| PUFA | 15.1±0.1 | 18.4±1.7c | 18.1±0.3c | 19.4±0.6c | 21.1±2.0c | 23.8±2.3c | 20.7±1.1c |
| SFA/MUFA | 0.9±0.0 | 1.1±0.1c | 1.2±0.0c | 1.2±0.1c | 1.2±0.1c | 1.0±0.1 | 1.4±0.1c |
| Tot. Omega 6 | 10.8±0.1 | 14.7±1.3c | 15.1±0.4c | 16.9±0.9c | 18.1±1.7c | 19.9±2.0c | 18.1±0.9c |
| Tot. Omega 3# | 4.2±0.6 | 3.7±0.4 | 3.0±0.6 | 2.5±0.7d | 2.5±0.5d | 3.9±0.3 | 2.6±0.5d |
Controls are the mean of cells cultured from 0 to 3 hours in the absence of fatty acid supplementation. The values are reported as % rel of the total fatty acid peak areas detected in the GC analysis. They are mean values ± SD of the n repetitions of the same experiment.
FAME are obtained from total lipid extraction, derivatization, and GC analysis.
The identification of the peaks have been performed by authentic samples and the identified peaks accounted for >98% of the total peaks.
Values higher than untreated control (*P = 0.01).
Values lower than untreated control (*P = 0.01).
Values higher than untreated control (*P<0.04).
Values lower than untreated control (*P<0.04).
Evaluated with standard compounds (mono-trans arachidonic acid isomers) obtained following references [12].
This value includes EPA and DHA.