Table 1. BCPreds prediction of B-cell epitopes from asparaginases.
| Epitope sequences of L-asparaginases | |||
|---|---|---|---|
| Amino acid position |
BCPred epitope sequence |
BCPred score |
|
| Rhizobium etli L-asparaginase | |||
| 186 |
TDGCNLPTPAFP |
0.988 |
|
| 150 |
GAGTDGYHLPDH |
0.970 |
|
| 338 |
GVTTGGVSFPFK |
0.929 |
|
| 35 |
YALGNPTRMTLA |
0.813 |
|
| Escherichia coli L-asparaginase | |||
|---|---|---|---|
| 7 |
LATGGTIAGGGD |
1 |
|
| 104 |
KCDKPVVMVGAM |
0.99 |
|
| 242 |
AGVGNGNLYKSV |
0.984 |
|
| 73 |
INTDCDKTDGFV |
0.945 |
|
| 159 |
DVTKTNTTDVAT |
0.898 |
|
| 58 |
SQDMNDNVWLTL |
0.891 |
|
| 122 |
SADGPFNLYNAV |
0.81 |
|
| Erwinia chrysantemi L-asparaginase | |||
|---|---|---|---|
| 312 |
TRTSDPKVIQEY |
0.991 |
|
| 108 |
TVKSDKPVVFVA |
0.983 |
|
| 277 |
TRTGNGIVPPDE |
0.972 |
|
| 21 |
ATGTQTTGYKAG |
0.958 |
|
| 244 |
GIVYAGMGAGSV |
0.934 |
|
| 264 |
RKALEKGVVVMR |
0.898 |
|
| 223 |
LYGYQDDPEYLY |
0.883 |
|
| 8 | VILATGGTIAGS | 0.802 | |
Epitope length: 12 amino acids, and specificity at 75%. Only BCPRED score values ≥ 0.8 were considered.