Figure 3. Luciferase inhibition by Isoflurane is avoided by substrate administration before anesthesia onset.

a, b) Difference in PE_max and AUC under pre/post Isoflurane conditions becomes more pronounced with increasing substrate concentration. c) The order of application between Isoflurane anesthesia and substrate had no impact on the time-to-peak for 15, 150 and 300 mg/kg D-Luciferin. (* statistically significant difference with p≤0.05 to standard protocol 150 mg/kg post-Iso in post hoc comparison with Sidak correction;+statistically significant difference with p≤0.05 between pre and post condition in post hoc comparison with Sidak correction).