Table 1. Primer sequences utilized in RT-qPCR evaluation of gene expression.

Domain Name	Sequence (F-forward 5′; R–reverse 5′)	Amplicon length (bp)	Primer efficiency	R2
Akt2	F-AGGACCCCATGGACTACAAGT-3′ R-CCAGGAGTTTGAGATAGTCGAA-3′	126	95%	0.99
APC	F- GACCAGAAGGCAATTGGAATA-3′ R- GGACTGCAAAAGCTGTCGTAT-3′	160	99%	0.98
Axin1	F-GATCTTCCGGGACAAAGAAG-3′ R-GCATGGTAGGGTCTTGAATGA-3′	147	99%	0.98
β-catenin	F-CCCTGAACTGACAAAACTGCTA-3′ R-AATAGCAGACACCATCTGAGGA-3′	136	98%	0.99
CKIα	F-TTGGGCGTCACTGTAATAAGTT-3′ R-GCAGTGCCAGTGAGATTTTTAT-3′	118	96%	0.99
Dsh	F-ACAACGAGACCAGCACAGAGT-3′ R-GTGTTGTCATCCTCGTCTGAGT-3′	205	101%	0.99
E-cadherin	F-CATTGTGTACTCCATCCTCAGC-3′ R-TGTACTTAAGCCCTCACCTTGA-3′	175	99%	0.99
FZD4	F-CTGCTGTTGCTGTTGCTACTC-3′ R-GATGAGAGGCGTGAAAGTTGT-3′	189	96%	0.98
GSK3β	F-ATCTGCCATCGGGATATTAAAC-3′ R-ATACGAAACATTCGGTTCTCCT-3′	120	102%	0.97
Integrin α6	F-AGTCTGCACATCTTCTTCCTGA-3′ R-CGCTCCAATCACTATATCTTGC-3′	122	100%	0.99
Integrin β4	F-GATGTGAATGAGTTCCGGAGT-3′ R-GTCAGCCTCGTAGTGGAAGG-3′	183	100%	0.99
LRP6	F-GCCAAATGGACTAACTTTGGAT-3′ R-GTGTCCTCAAATAACGTCAAGG-3′	159	102%	0.99
PP1	F-AAAACCTTCACCGACTGCTT-3′ R-ATACGCCGAATCTGTTCCAT-3′	119	97%	0.99
TCF4	F-TTCTTTCAGCCAACAGACATTC-3′ R-AGTTGCAGACTGGACAGGAAG-3	128	101%	0.99
Versican	F-CCTGCAATTACCATCTCACCTA-3′ R -CAGGGAGTTGATTTCATAACGA-3′	122	92.1%	0.99
Vimentin	F-ACGTTCGTCAGCAGTATGAAA-3′ R-GTTAGCAGCCTCAGAGAGGTC-3′	98	97%	0.99
Wnt4	F-AGCTGGAAAAGTGTGGCTGT-3′ R-GTCCACAAACGACTGTGAGAAG-3′	119	103%	0.97