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. 2013 Feb 6;8(2):e56025. doi: 10.1371/journal.pone.0056025

Table 3. Antibodies used for Immunofluorescent Localization.

Primary Antibody Host Species & Epitope Dilution Company
anti-β-catenin Rabbit polyclonal to aa 768–781 of human or mouse β-catenin 1∶100 AbCam ab6302
anti-E-cadherin Mouse monoclonal[M168] to C terminal region of mouse E-cadherin 1∶100 AbCam ab76055
anti-FZD4 Rabbit polyclonal to 488–537 of human Frizzled 4 1∶100 AbCam ab83024
anti-Integrin β4 Mouse IgG1 to human integrin β4 aa. 1612–1821 1∶50 BD #611232
anti-Laminin Rabbit polyclonal to laminin 1∶100 AbCam ab11575
anti-Wnt4 Rabbit polyclonal to the center region of human Wnt4 1∶50 AbCam ab91226
anti-Vimentin Rabbit polyclonal to C-terminus of human vimentin 1∶100 AbCam ab45939
Secondary Antibody
anti-Mouse Ig Donkey polyclonal to mouse IgG H&L – conjugated with a fluorochrome with EX/EM* of 591/616 nm 1∶200 Jackson ImmunoResearch #715-515-150
anti-Mouse Ig Donkey polyclonal to mouse IgG H&L – conjugated with a fluorochrome with EX/EM* of 493/518 nm 1∶200 Jackson ImmunoResearch #715-485-150
anti-Rabbit Ig Donkey polyclonal to rabbit IgG H&L – conjugated with a flurochrome with Ex/EM* of 591/616 nm 1∶200 Jackson ImmunoResearch #711-515-152
anti-Rabbit Ig Donkey polyclonal to rabbit IgG H&L – conjugated with a fluorochrome with EX/EM* of 493/518 nm 1∶200 Jackson ImmunoResearch #711-485-152