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. 2013 Feb 6;8(2):e56203. doi: 10.1371/journal.pone.0056203

Figure 5. Chaperone activity modulated the formation of intracellular bodies of purine biosynthetic enzymes.

Figure 5

(A) Short-term treatment with the HSP90 chaperone inhibitor geldanamycin induced puncta formation in a concentration-dependent manner, shown here for PPAT-EGFP in HEK293T cells. Bars indicate average +/- 1 s. d. across 3 replicates, n  =  680, 351, 565, 601, and 616 total cells, respectively. (B) Low-dosage geldanamycin pre-treatment—known to induce HSP70 activity [11]—suppressed oxidatively-induced puncta formation, shown here for PPAT-EGFP in HEK293T cells. Bars indicate average +/- 1 s. d. across 3 replicates, n  =  555, 500, 608, and 601 total cells, respectively. Results were similar for FGAMS-EGFP in the absence of hydrogen peroxide (data not shown).