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. 2013 Feb 6;8(2):e55868. doi: 10.1371/journal.pone.0055868

Figure 6. Effect of oral tempol on dimer-sized SOD1 levels in spinal cord homogenates of G93A rats.

Figure 6

Relative quantification of the levels of dimer-sized SOD1 species in spinal cord homogenates of G93A rats both treated and untreated with oral tempol. A) Western blot analysis of spinal cord homogenates obtained at the symptomatic phase compared with that of wild-type rats. The blots were visualized with an anti-SOD1 antibody; the dimer-sized SOD1 species that was used as standard was obtained through the enzymés peroxidase activity as previously described (48). Female rats received tempol (30 mM) in their drinking water starting at 7 weeks of age. B) The relative quantification of the dimer-sized SOD1 species by densitometry of the respective bands shown in A. The values shown correspond to the mean ± standard error of the results obtained with homogenates from 3 different rats from each group: wild-type, untreated G93A and treated G93A; *p<0.05 (one-way ANOVA).