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. 2013 Feb 6;8(2):e54712. doi: 10.1371/journal.pone.0054712

Figure 1. Novel murine anti-CD20 administration preferentially depletes follicular but not marginal zone B-cells.

Figure 1

Eight week old female NOD mice were treated with 50 µg anti-CD20 and 7 days later various B-cell subsets in the spleen were analyzed. Gating strategy is shown in the top panel. Lymphocytes were gated on (A) B220+ (total B-cells) and further classified as FO B-cells (B220+ IgMhi, CD21int), immature B-cells (B220+ IgMhi CD21−), and MZ B-cells (B220+ IgMhi CD21hi). Total B220+ B-cell numbers were reduced in the spleen with significant reductions observed in number of follicular and immature B-cells but not MZ B-cells (B). Eight week old female NOD mice were given anti-CD20 i.v. Seven days post anti-CD20 treatment, frequencies of B- and T-cells in peripheral blood were determined by staining with anti-IgM/B220 for B-cells or anti-CD4/CD8 for T-cells; loss of circulating B-cells was associated with compensatory increases in CD4+ and CD8+ T-cell frequencies in NOD mice (C). Frequencies of circulating B-cells returned to similar levels as in untreated NOD mice by 60 days post-treatment (D). Data are means ± SEM. Representative data from 3 independent experiments with similar results are shown. Statistical analysis was performed using unpaired students t-test using Graphpad Prism software.