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. 2013 Feb 6;8(2):e55920. doi: 10.1371/journal.pone.0055920

Figure 5. Mapping the hGBP1-binding region of NS1.

Figure 5

A. Schematic representation of Flag-tagged wild-type NS1 and truncated mutants. B. H1299 cells were transfected with the indicated plasmids in the presence of plasmid Myc-hGBP1-wt. Transfectants were collected after 36 h and immunoprecipitated with anti-Myc antibody. C. H1299 cells were transfected with plasmid Flag-NS1-wt or Flag-NS1-Δ123/144 in the presence of plasmid Myc-hGBP1-wt. Transfectants were collected after 36 h and immunoprecipitated with anti-Myc antibody. IP, immunoprecipitation. IB, western blot.