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. 2013 Feb 6;8(2):e55511. doi: 10.1371/journal.pone.0055511

Figure 7. Phosphorylation of Perilipin 1A and HSL in response to FSK and L-γ-MSH.

Figure 7

3T3L1 adipocytes were exposed to control medium, 6 µM forskolin (F), or 200 nM L-γ-MSH (M) for 1, 5, or 20 minutes, then fixed and co-labeled with either anti-pPeri-site 5+ anti-pHSL-serine 563, or anti-pPeri-site 6+ anti-pHSL-serine 660. Images were obtained with a 20X objective (4 images/well, representing an average of 930 cells/well). A and B represent Area Pm values obtained for pPeri-site 5 and pHSL-serine 563. C and D represent Area Pm values obtained for pPeri-site 6 and pHSL-serine 660, respectively. Each bar represents the mean ± SD for n = 7 to 8 wells. ** p<0.01 compared to controls (ANOVA followed by Tukey’s test); *** p<0.001 compared to controls. a p<0.05 vs. controls and M; b p<0.05 vs. controls and F.