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. 2013 Feb 6;8(2):e54974. doi: 10.1371/journal.pone.0054974

Figure 6. Antiplasmodial effect of a pantothenamide and pantothenol in fresh and aged human serum-complete RPMI.

Figure 6

The concentration-response curves show the effect of increasing concentrations of compound 12 (A) and pantothenol (B) on the proliferation of P. falciparum parasites cultured (for 96 h) in human serum-complete RPMI as measured using the SYBR Green I-based growth assay. Assays were performed using human serum-complete RPMI prepared immediately prior to experimentation (fresh; open circles) or human serum-complete RPMI heated at 37°C for 40 h immediately following preparation (aged; closed circles). The data obtained with parasites cultured in fresh human serum-complete RPMI are from three independent experiments, each performed in duplicate or triplicate, and error bars represent SEM. The data obtained with parasites cultured in aged human serum-complete RPMI are from two independent experiments, each performed in duplicate or triplicate, and error bars represent range/2. For clarity, in B, concentration-response curves represented by the closed circles are shown with positive error bars only, and the concentration-response curves represented by the open circles are shown with negative error bars only. Where not shown, error bars are smaller than the symbol.