Figure 6. Effect of LC06 and LC08 treatment on VEGF-induced corneal angiogenesis.

(A) Angiogenesis was induced by implanting VEGF (300 ng) or vehicle soaked nylaflo discs into the cornea of BALB/c mice. PBS control did not induce vessel outgrowth from the limbus to the disc compared to VEGF positive control. LC06, LC08 and bevacizumab were injected with 10 mg/kg i.v. at the day of disc implantation. Treatment with both anti-Ang-2 antibodies significantly inhibited neoangiogenesis in a comparable range as bevacizumab (n = 5, *p<0.001 compared to VEGF control, Student's t-test). (B) Representative pictures of vehicle, LC06 and LC08 treated mice. The results were confirmed in 3 additional independent experiments. Results are expressed as mean ± SEM.