Figure 7. Validation of RNA-Seq results using RT-PCR. A:

Gel electrophoresis of the amplified PCR products from cDNA samples (+) of brain, breast, colon, kidney, lung and testis. The RNA of the investigated tissues does not contain genomic DNA contamination, as shown in (−). The presence of broadly expressed ORs detected by RNA-Seq could be confirmed by RT-PCR. B: Table showing the summarized PCR validation in comparison to RNA-Seq data. We investigated 26 different ORs that showed broad or high expression. Green color indicates the detection of ORs with the respective technique; red color indicates no detection.