Table 1. Identification of protein candidates showing significant changes in expression level in response to anti-CaM treatment.
ID. | Proteins identified | Accession No. | Organism | NCBI nr database | ||
Mascot score | Number of matched peptides | Sequence coverage | ||||
17 KDa | Glycine-rich RNA-binding protein | gi|4704605 | Picea glauca | 401 | 6 | 48% |
21 KDa | Unknown (putative cyclophilin) | gi|116779193 | Picea sitchensis | 136 | 4 | 15% |
25 KDa | Unknown (putative translationally controlled tumor protein) | gi|116782446 | Picea sitchensis | 208 | 4 | 33% |
26 KDa | Unknown (putative phenylcoumaran benzylic ether reductase) | gi|116784723 | Picea sitchensis | 656 | 10 | 51% |
ID corresponds to protein bands indicated in Figure 5. Accession No. represents protein identified by MS and extracted from the National Center for Biotechnology Information (NCBI) database (in Aug, 2012). Mowse scores greater than 47 were considered significant (p<0.05); threshold score for individual ion was no less than 15.