Figure 4. Glucose 6P binds to HlyIIR to enhance its DNA binding capacity.

(A) Isothermal titration calorimetry (ITC) assays were performed to measure heat variations generated upon binding between glucose 6P (G6P) and HlyIIR (red line) or arabinose and HlyIIR (blue squares). (B) ITC assays were performed to measure binding between a DNA fragment containing the HlyIIR binding site and HlyIIR supplemented either with glucose 6P (red line) or with arabinose (blue line).