Table 2. Primers for real-time PCR.

Target group	AnnealingTemp (°C)	Primer name and sequence (5′–3′ direction)	Reference
All bacteria	53	Eub338F: ACT CCT ACG GGA GGC AGC AG	[8]
		Eub518R: ATT ACC GCG GCT GCT GG	[49]
Alphaproteobacteria	60	Eub338F: ACT CCT ACG GGA GGC AGC AG	[8]
		Alf685R: TCT ACG RAT TTC ACC YCT AC	[8]
Betaproteobacteria	60	Eub338F: ACT CCT ACG GGA GGC AGC AG	[8]
		Bet680R: TCA CTG CTA CAC GYG	[50]
Actinobacteria	60	Actino235F: CGC GGC CTA TCA GCT TGT TG	[51]
		Eub518R: ATT ACC GCG GCT GCT GG	[49]
Bacteroidetes	60	Cfb319F: GTA CTG AGA CAC GGA CCA	[52]
		Eub518R: ATT ACC GCG GCT GCT GG	[49]
Firmicutes	60	Lgc353F: GCA GTA GGG AAT CTT CCG	[53]
		Eub518R: ATT ACC GCG GCT GCT GG	[49]
Cyanobacteria	60	Cya106F: CGG ACG GGT GAG TAA CGC GTG A	[54]
		Cya359R: CCC ATT GCG GAA RAT TCC CC	[54]
Archaea	56	Arch16F: CTG GTT GAT CCT GCC AG	[55]
		Arch344R: TTC GCG CCT GST GCR CCC CG	[56]
Crenarcheota	56	ArchGI334F: AGA TGG GTA CTG AGA CAC GGA C	[25]
		ArchGI554R: CTG TAG GCC CAA TAA TCA TCC T	[25]