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XZ reconstruction of confocal stacks of a monolayer of NHE3’38HA3 cells labeled with ezrin (red), surface NHE3 (green) and total NHE3 (blue) after treatment with non-targeting siRNA A) or siRNA targeted against ezrin B). C) Quantification by an immunoperoxidase based assay of surface and total NHE3 after treatment with non-targeting siRNA or siRNA targeted against ezrin (n = 4 per condition).
